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flow cytometry anti mouse cd3e apc  (Biogems International)


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    Biogems International flow cytometry anti mouse cd3e apc
    PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow <t>cytometry.</t> Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.
    Flow Cytometry Anti Mouse Cd3e Apc, supplied by Biogems International, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry anti mouse cd3e apc/product/Biogems International
    Average 93 stars, based on 6 article reviews
    flow cytometry anti mouse cd3e apc - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Platycodon grandiflorum Triggers Antitumor Immunity by Restricting PD-1 Expression of CD8 + T Cells in Local Tumor Microenvironment"

    Article Title: Platycodon grandiflorum Triggers Antitumor Immunity by Restricting PD-1 Expression of CD8 + T Cells in Local Tumor Microenvironment

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2022.774440

    PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow cytometry. Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.
    Figure Legend Snippet: PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow cytometry. Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.

    Techniques Used: Gene Expression, Flow Cytometry, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction

    PG decreases PD-1 through VEGF-A–VEGFR-2 axis. (A) Flow cytometer detection of PD-1 expression in CD8 + T cells after treatment with PG. (B) Changes of VEGF-A transcription level in LLC after PG treatment. (C) Western blot analysis of the effect of PG on the expression of VEGF-A protein. (D) The proportion of PD-1 + CD8 + T cells in CD8 + T cells was tested by flow cytometry. Treatment of CD8 + T cells includes different tumor-conditioned media (TCM-C, TCM-1, TCM-5, and TCM-10) groups, and the blank control group and VEGF-A group. The % PD-1 + CD8 + T cells were quantified and are presented in the right panel. (E) The ratio of CD8 + T cells proliferation under different treatments: tumor-conditioned media (TCM-C), TCM-1, TCM-5, TCM-10, blank control group, and VEGF-A group. The % proliferative CD8 + T cells were quantified and are presented in the right panel. (F) Transcription levels of VEGFR-1 and VEGFR-2 were detected by RT-qPCR. Bar chart shows quantification of transcriptional levels compared to GAPDH control in each condition. The results are presented as the mean ± SD of triplicate determination. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: PG decreases PD-1 through VEGF-A–VEGFR-2 axis. (A) Flow cytometer detection of PD-1 expression in CD8 + T cells after treatment with PG. (B) Changes of VEGF-A transcription level in LLC after PG treatment. (C) Western blot analysis of the effect of PG on the expression of VEGF-A protein. (D) The proportion of PD-1 + CD8 + T cells in CD8 + T cells was tested by flow cytometry. Treatment of CD8 + T cells includes different tumor-conditioned media (TCM-C, TCM-1, TCM-5, and TCM-10) groups, and the blank control group and VEGF-A group. The % PD-1 + CD8 + T cells were quantified and are presented in the right panel. (E) The ratio of CD8 + T cells proliferation under different treatments: tumor-conditioned media (TCM-C), TCM-1, TCM-5, TCM-10, blank control group, and VEGF-A group. The % proliferative CD8 + T cells were quantified and are presented in the right panel. (F) Transcription levels of VEGFR-1 and VEGFR-2 were detected by RT-qPCR. Bar chart shows quantification of transcriptional levels compared to GAPDH control in each condition. The results are presented as the mean ± SD of triplicate determination. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Flow Cytometry, Expressing, Western Blot, Control, Quantitative RT-PCR

    PG downregulates VEGF-A by p-STAT3 signal. (A) Protein levels of STAT3 and P-STAT3 in WT and PG-treated groups. (B) Protein expressions of STAT3 and P-STAT3 after treated by PG were tested by Western Blot analysis. Bar chart shows quantification of protein levels compared to GAPDH control in each condition. (C) The transcriptional level of the key downstream factors of STAT3: IL-10, IL-6, and VEGF-A in tissues of control and PG-treated groups. (D) Apoptosis-inducing effect of PD on LLC cells. Annexin V/propidium iodide (PI) staining using flow cytometry was performed after LLC cells were treated with various concentrations of PG for 48 h. Taxol at concentration of 250 nM was used as positive control and DMSO (0.05%) without PG was used as negative control. Apoptosis and necrosis were quantified in the right histogram as the percentage of apoptosis. Quantitative analysis of the results of flow cytometry analysis presented the mean ± standard deviation of triplicate. (E) Cell cycle distribution was assayed with PI staining by flow cytometry after 24 h of treatment with or without PG. Percentage of cells in G1, S, or G2 phases of the cell cycle presented in the right bar chart. Data are presented as the mean ± standard deviation of triplicates. (F) Effect of different concentrations of PG on the proliferation in LLC cells was measured by a colony formation assay. (G) The expression of apoptosis-related proteins, cell cycle‐related proteins and cell cycle‐related proteins in LLC cells treated with or without PG and Taxol was estimated by western blot analysis after 48 h (From left to right). All quantitative data are presented as the mean ± standard deviation of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: PG downregulates VEGF-A by p-STAT3 signal. (A) Protein levels of STAT3 and P-STAT3 in WT and PG-treated groups. (B) Protein expressions of STAT3 and P-STAT3 after treated by PG were tested by Western Blot analysis. Bar chart shows quantification of protein levels compared to GAPDH control in each condition. (C) The transcriptional level of the key downstream factors of STAT3: IL-10, IL-6, and VEGF-A in tissues of control and PG-treated groups. (D) Apoptosis-inducing effect of PD on LLC cells. Annexin V/propidium iodide (PI) staining using flow cytometry was performed after LLC cells were treated with various concentrations of PG for 48 h. Taxol at concentration of 250 nM was used as positive control and DMSO (0.05%) without PG was used as negative control. Apoptosis and necrosis were quantified in the right histogram as the percentage of apoptosis. Quantitative analysis of the results of flow cytometry analysis presented the mean ± standard deviation of triplicate. (E) Cell cycle distribution was assayed with PI staining by flow cytometry after 24 h of treatment with or without PG. Percentage of cells in G1, S, or G2 phases of the cell cycle presented in the right bar chart. Data are presented as the mean ± standard deviation of triplicates. (F) Effect of different concentrations of PG on the proliferation in LLC cells was measured by a colony formation assay. (G) The expression of apoptosis-related proteins, cell cycle‐related proteins and cell cycle‐related proteins in LLC cells treated with or without PG and Taxol was estimated by western blot analysis after 48 h (From left to right). All quantitative data are presented as the mean ± standard deviation of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Western Blot, Control, Staining, Flow Cytometry, Concentration Assay, Positive Control, Negative Control, Standard Deviation, Colony Assay, Expressing



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    flow cytometry anti mouse cd3e apc  (Biogems International)
    93
    Biogems International flow cytometry anti mouse cd3e apc
    PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow <t>cytometry.</t> Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.
    Flow Cytometry Anti Mouse Cd3e Apc, supplied by Biogems International, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometry anti mouse cd3e apc/product/Biogems International
    Average 93 stars, based on 1 article reviews
    flow cytometry anti mouse cd3e apc - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow cytometry. Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.

    Journal: Frontiers in Pharmacology

    Article Title: Platycodon grandiflorum Triggers Antitumor Immunity by Restricting PD-1 Expression of CD8 + T Cells in Local Tumor Microenvironment

    doi: 10.3389/fphar.2022.774440

    Figure Lengend Snippet: PG enhances antitumor immunity via reducing PD-1 on the surface of CD8 + T. (A) The heat map of Pearson correlation coefficients (PCCs) evaluated by the four methods between the gene expression level of PG targets and immune phenotypes; Benjamini–Hochberg (BH) adjusted p -values of PCCs <0.05 are shown in white. (B) LLC tumor infiltrating CD8 + T cells in PG-treated versus WT mice analyzed by flow cytometry. Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting CD8 + T cells (CD3+CD8+). Representative dot plots with indicated percentages of CD8 + T cells populations within CD45 + cells in LLC tumors from the WT and PG-treated groups, respectively. (C) Protein level of IFN-γ and granzyme B in WT and PG-treated groups by means of western blotting. (D) Transcription levels of IFN-γ and granzyme B in WT and PG-treated groups by means of RT-PCR. (E) Protein level of PD-1 in WT and PG-treated groups. (F) Flow cytometry analysis of single-cell suspensions obtained from LLC tumors and stained for detecting PD-1 + CD8 + T cells. Representative dot plots with indicated percentages of PD-1 + CD8 + T-cell populations within CD8 + T cells in LLC tumors from WT and PG-treated groups, respectively. All plots of flow cytometry analysis were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. ** p < 0.01, *** p < 0.001 compared with WT by unpaired t-test; n > = 3.

    Article Snippet: The untouched and highly purified mouse CD8 + T cells were isolated from single-cell suspensions of splenocytes by immunomagnetic negative selection using the EasySep Mouse CD8 + T-Cell Isolation Kit (19853, STEMCELL), according to the manufacturer’s instructions, and the CD8 + T cells purity (>90%) was identified by flow cytometry Anti-mouse CD3e APC (05122-80-25, Biogems, PeproTech) and anti-mouse CD8a PE (100707, Biolegend).

    Techniques: Gene Expression, Flow Cytometry, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction

    PG decreases PD-1 through VEGF-A–VEGFR-2 axis. (A) Flow cytometer detection of PD-1 expression in CD8 + T cells after treatment with PG. (B) Changes of VEGF-A transcription level in LLC after PG treatment. (C) Western blot analysis of the effect of PG on the expression of VEGF-A protein. (D) The proportion of PD-1 + CD8 + T cells in CD8 + T cells was tested by flow cytometry. Treatment of CD8 + T cells includes different tumor-conditioned media (TCM-C, TCM-1, TCM-5, and TCM-10) groups, and the blank control group and VEGF-A group. The % PD-1 + CD8 + T cells were quantified and are presented in the right panel. (E) The ratio of CD8 + T cells proliferation under different treatments: tumor-conditioned media (TCM-C), TCM-1, TCM-5, TCM-10, blank control group, and VEGF-A group. The % proliferative CD8 + T cells were quantified and are presented in the right panel. (F) Transcription levels of VEGFR-1 and VEGFR-2 were detected by RT-qPCR. Bar chart shows quantification of transcriptional levels compared to GAPDH control in each condition. The results are presented as the mean ± SD of triplicate determination. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Platycodon grandiflorum Triggers Antitumor Immunity by Restricting PD-1 Expression of CD8 + T Cells in Local Tumor Microenvironment

    doi: 10.3389/fphar.2022.774440

    Figure Lengend Snippet: PG decreases PD-1 through VEGF-A–VEGFR-2 axis. (A) Flow cytometer detection of PD-1 expression in CD8 + T cells after treatment with PG. (B) Changes of VEGF-A transcription level in LLC after PG treatment. (C) Western blot analysis of the effect of PG on the expression of VEGF-A protein. (D) The proportion of PD-1 + CD8 + T cells in CD8 + T cells was tested by flow cytometry. Treatment of CD8 + T cells includes different tumor-conditioned media (TCM-C, TCM-1, TCM-5, and TCM-10) groups, and the blank control group and VEGF-A group. The % PD-1 + CD8 + T cells were quantified and are presented in the right panel. (E) The ratio of CD8 + T cells proliferation under different treatments: tumor-conditioned media (TCM-C), TCM-1, TCM-5, TCM-10, blank control group, and VEGF-A group. The % proliferative CD8 + T cells were quantified and are presented in the right panel. (F) Transcription levels of VEGFR-1 and VEGFR-2 were detected by RT-qPCR. Bar chart shows quantification of transcriptional levels compared to GAPDH control in each condition. The results are presented as the mean ± SD of triplicate determination. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The untouched and highly purified mouse CD8 + T cells were isolated from single-cell suspensions of splenocytes by immunomagnetic negative selection using the EasySep Mouse CD8 + T-Cell Isolation Kit (19853, STEMCELL), according to the manufacturer’s instructions, and the CD8 + T cells purity (>90%) was identified by flow cytometry Anti-mouse CD3e APC (05122-80-25, Biogems, PeproTech) and anti-mouse CD8a PE (100707, Biolegend).

    Techniques: Flow Cytometry, Expressing, Western Blot, Control, Quantitative RT-PCR

    PG downregulates VEGF-A by p-STAT3 signal. (A) Protein levels of STAT3 and P-STAT3 in WT and PG-treated groups. (B) Protein expressions of STAT3 and P-STAT3 after treated by PG were tested by Western Blot analysis. Bar chart shows quantification of protein levels compared to GAPDH control in each condition. (C) The transcriptional level of the key downstream factors of STAT3: IL-10, IL-6, and VEGF-A in tissues of control and PG-treated groups. (D) Apoptosis-inducing effect of PD on LLC cells. Annexin V/propidium iodide (PI) staining using flow cytometry was performed after LLC cells were treated with various concentrations of PG for 48 h. Taxol at concentration of 250 nM was used as positive control and DMSO (0.05%) without PG was used as negative control. Apoptosis and necrosis were quantified in the right histogram as the percentage of apoptosis. Quantitative analysis of the results of flow cytometry analysis presented the mean ± standard deviation of triplicate. (E) Cell cycle distribution was assayed with PI staining by flow cytometry after 24 h of treatment with or without PG. Percentage of cells in G1, S, or G2 phases of the cell cycle presented in the right bar chart. Data are presented as the mean ± standard deviation of triplicates. (F) Effect of different concentrations of PG on the proliferation in LLC cells was measured by a colony formation assay. (G) The expression of apoptosis-related proteins, cell cycle‐related proteins and cell cycle‐related proteins in LLC cells treated with or without PG and Taxol was estimated by western blot analysis after 48 h (From left to right). All quantitative data are presented as the mean ± standard deviation of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Platycodon grandiflorum Triggers Antitumor Immunity by Restricting PD-1 Expression of CD8 + T Cells in Local Tumor Microenvironment

    doi: 10.3389/fphar.2022.774440

    Figure Lengend Snippet: PG downregulates VEGF-A by p-STAT3 signal. (A) Protein levels of STAT3 and P-STAT3 in WT and PG-treated groups. (B) Protein expressions of STAT3 and P-STAT3 after treated by PG were tested by Western Blot analysis. Bar chart shows quantification of protein levels compared to GAPDH control in each condition. (C) The transcriptional level of the key downstream factors of STAT3: IL-10, IL-6, and VEGF-A in tissues of control and PG-treated groups. (D) Apoptosis-inducing effect of PD on LLC cells. Annexin V/propidium iodide (PI) staining using flow cytometry was performed after LLC cells were treated with various concentrations of PG for 48 h. Taxol at concentration of 250 nM was used as positive control and DMSO (0.05%) without PG was used as negative control. Apoptosis and necrosis were quantified in the right histogram as the percentage of apoptosis. Quantitative analysis of the results of flow cytometry analysis presented the mean ± standard deviation of triplicate. (E) Cell cycle distribution was assayed with PI staining by flow cytometry after 24 h of treatment with or without PG. Percentage of cells in G1, S, or G2 phases of the cell cycle presented in the right bar chart. Data are presented as the mean ± standard deviation of triplicates. (F) Effect of different concentrations of PG on the proliferation in LLC cells was measured by a colony formation assay. (G) The expression of apoptosis-related proteins, cell cycle‐related proteins and cell cycle‐related proteins in LLC cells treated with or without PG and Taxol was estimated by western blot analysis after 48 h (From left to right). All quantitative data are presented as the mean ± standard deviation of the mean. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The untouched and highly purified mouse CD8 + T cells were isolated from single-cell suspensions of splenocytes by immunomagnetic negative selection using the EasySep Mouse CD8 + T-Cell Isolation Kit (19853, STEMCELL), according to the manufacturer’s instructions, and the CD8 + T cells purity (>90%) was identified by flow cytometry Anti-mouse CD3e APC (05122-80-25, Biogems, PeproTech) and anti-mouse CD8a PE (100707, Biolegend).

    Techniques: Western Blot, Control, Staining, Flow Cytometry, Concentration Assay, Positive Control, Negative Control, Standard Deviation, Colony Assay, Expressing